PhanerochaeteV1, Main, Exploration, bibRecord, 000222

Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.

Identifieur interne : 000222 ( Main/Exploration ); précédent : 000221; suivant : 000223

Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.

Auteurs : Carolyn A. Zeiner [États-Unis] ; Samuel O. Purvine [États-Unis] ; Erika M. Zink [États-Unis] ; Ljiljana Paša-Toli [États-Unis] ; Dominique L. Chaput [États-Unis] ; Sajeet Haridas [États-Unis] ; Si Wu [États-Unis] ; Kurt Labutti [États-Unis] ; Igor V. Grigoriev [États-Unis] ; Bernard Henrissat [France, Arabie saoudite] ; Cara M. Santelli [États-Unis] ; Colleen M. Hansel [États-Unis]

Source :

PloS one [ 1932-6203 ] ; 2016.

RBID : pubmed:27434633

Descripteurs français

KwdFr :
- Annotation de séquence moléculaire (MeSH), Ascomycota (enzymologie), Biologie informatique (MeSH), Carbone (métabolisme), Cations divalents (MeSH), Cellulases (classification), Cellulases (métabolisme), Dépollution biologique de l'environnement (MeSH), Glycosidases (classification), Glycosidases (métabolisme), Lignine (métabolisme), Manganèse (métabolisme), Microbiologie du sol (MeSH), Oxydoréduction (MeSH), Polygalacturonase (classification), Polygalacturonase (métabolisme), Radical hydroxyle (métabolisme), Spécificité d'espèce (MeSH).
MESH :
- classification : Cellulases, Glycosidases, Polygalacturonase.
- enzymologie : Ascomycota.
- métabolisme : Carbone, Cellulases, Glycosidases, Lignine, Manganèse, Polygalacturonase, Radical hydroxyle.
- Annotation de séquence moléculaire, Biologie informatique, Cations divalents, Dépollution biologique de l'environnement, Microbiologie du sol, Oxydoréduction, Spécificité d'espèce.

English descriptors

KwdEn :
- Ascomycota (enzymology), Biodegradation, Environmental (MeSH), Carbon (metabolism), Cations, Divalent (MeSH), Cellulases (classification), Cellulases (metabolism), Computational Biology (MeSH), Glycoside Hydrolases (classification), Glycoside Hydrolases (metabolism), Hydroxyl Radical (metabolism), Lignin (metabolism), Manganese (metabolism), Molecular Sequence Annotation (MeSH), Oxidation-Reduction (MeSH), Polygalacturonase (classification), Polygalacturonase (metabolism), Soil Microbiology (MeSH), Species Specificity (MeSH).
MESH :
- chemical , classification : Cellulases, Glycoside Hydrolases, Polygalacturonase.
- chemical , metabolism : Carbon, Cellulases, Glycoside Hydrolases, Hydroxyl Radical, Lignin, Manganese, Polygalacturonase.
- enzymology : Ascomycota.
- Biodegradation, Environmental, Cations, Divalent, Computational Biology, Molecular Sequence Annotation, Oxidation-Reduction, Soil Microbiology, Species Specificity.

Abstract

Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the environment. While secretome studies of model organisms such as Phanerochaete chrysosporium and Aspergillus species have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates or conducted side-by-side comparisons of diverse species. Thus, the mechanisms of carbon degradation by many ubiquitous soil fungi remain poorly understood. Here we use a combination of LC-MS/MS, genomic, and bioinformatic analyses to characterize and compare the protein composition of the secretomes of four recently isolated, cosmopolitan, Mn(II)-oxidizing Ascomycetes (Alternaria alternata SRC1lrK2f, Stagonospora sp. SRC1lsM3a, Pyrenochaeta sp. DS3sAY3a, and Paraconiothyrium sporulosum AP3s5-JAC2a). We demonstrate that the organisms produce a rich yet functionally similar suite of extracellular enzymes, with species-specific differences in secretome composition arising from unique amino acid sequences rather than overall protein function. Furthermore, we identify not only a wide range of carbohydrate-active enzymes that can directly oxidize recalcitrant carbon, but also an impressive suite of redox-active accessory enzymes that suggests a role for Fenton-based hydroxyl radical formation in indirect, non-specific lignocellulose attack. Our findings highlight the diverse oxidative capacity of these environmental isolates and enhance our understanding of the role of filamentous Ascomycetes in carbon turnover in the environment.

DOI: 10.1371/journal.pone.0157844
PubMed: 27434633
PubMed Central: PMC4951024

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000204
to stream Main, to step Curation: 000204

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.</title>
<author><name sortKey="Zeiner, Carolyn A" sort="Zeiner, Carolyn A" uniqKey="Zeiner C" first="Carolyn A" last="Zeiner">Carolyn A. Zeiner</name>
<affiliation wicri:level="4"><nlm:affiliation>School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
<settlement type="city">Cambridge (Massachusetts)</settlement>
</placeName>
<orgName type="university">Université Harvard</orgName>
</affiliation>
</author>
<author><name sortKey="Purvine, Samuel O" sort="Purvine, Samuel O" uniqKey="Purvine S" first="Samuel O" last="Purvine">Samuel O. Purvine</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zink, Erika M" sort="Zink, Erika M" uniqKey="Zink E" first="Erika M" last="Zink">Erika M. Zink</name>
<affiliation wicri:level="2"><nlm:affiliation>Biological Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Biological Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pasa Toli, Ljiljana" sort="Pasa Toli, Ljiljana" uniqKey="Pasa Toli L" first="Ljiljana" last="Paša-Toli">Ljiljana Paša-Toli</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chaput, Dominique L" sort="Chaput, Dominique L" uniqKey="Chaput D" first="Dominique L" last="Chaput">Dominique L. Chaput</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, Washington, DC</wicri:regionArea>
<placeName><region type="state">District de Columbia</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Haridas, Sajeet" sort="Haridas, Sajeet" uniqKey="Haridas S" first="Sajeet" last="Haridas">Sajeet Haridas</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wu, Si" sort="Wu, Si" uniqKey="Wu S" first="Si" last="Wu">Si Wu</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Labutti, Kurt" sort="Labutti, Kurt" uniqKey="Labutti K" first="Kurt" last="Labutti">Kurt Labutti</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Henrissat, Bernard" sort="Henrissat, Bernard" uniqKey="Henrissat B" first="Bernard" last="Henrissat">Bernard Henrissat</name>
<affiliation wicri:level="3"><nlm:affiliation>Architecture et Fonction des Macromolécules Biologiques, UMR7257, Centre National de la Recherche Scientifique and Aix-Marseille Université, 13288 Marseille Cedex 9, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, UMR7257, Centre National de la Recherche Scientifique and Aix-Marseille Université, 13288 Marseille Cedex 9</wicri:regionArea>
<placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Department of Biological Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589</wicri:regionArea>
<wicri:noRegion>21589</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Santelli, Cara M" sort="Santelli, Cara M" uniqKey="Santelli C" first="Cara M" last="Santelli">Cara M. Santelli</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Earth Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Earth Sciences, University of Minnesota, Minneapolis, Minnesota</wicri:regionArea>
<placeName><region type="state">Minnesota</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hansel, Colleen M" sort="Hansel, Colleen M" uniqKey="Hansel C" first="Colleen M" last="Hansel">Colleen M. Hansel</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:27434633</idno>
<idno type="pmid">27434633</idno>
<idno type="doi">10.1371/journal.pone.0157844</idno>
<idno type="pmc">PMC4951024</idno>
<idno type="wicri:Area/Main/Corpus">000204</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000204</idno>
<idno type="wicri:Area/Main/Curation">000204</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000204</idno>
<idno type="wicri:Area/Main/Exploration">000204</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.</title>
<author><name sortKey="Zeiner, Carolyn A" sort="Zeiner, Carolyn A" uniqKey="Zeiner C" first="Carolyn A" last="Zeiner">Carolyn A. Zeiner</name>
<affiliation wicri:level="4"><nlm:affiliation>School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
<settlement type="city">Cambridge (Massachusetts)</settlement>
</placeName>
<orgName type="university">Université Harvard</orgName>
</affiliation>
</author>
<author><name sortKey="Purvine, Samuel O" sort="Purvine, Samuel O" uniqKey="Purvine S" first="Samuel O" last="Purvine">Samuel O. Purvine</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zink, Erika M" sort="Zink, Erika M" uniqKey="Zink E" first="Erika M" last="Zink">Erika M. Zink</name>
<affiliation wicri:level="2"><nlm:affiliation>Biological Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Biological Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pasa Toli, Ljiljana" sort="Pasa Toli, Ljiljana" uniqKey="Pasa Toli L" first="Ljiljana" last="Paša-Toli">Ljiljana Paša-Toli</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chaput, Dominique L" sort="Chaput, Dominique L" uniqKey="Chaput D" first="Dominique L" last="Chaput">Dominique L. Chaput</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, Washington, DC</wicri:regionArea>
<placeName><region type="state">District de Columbia</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Haridas, Sajeet" sort="Haridas, Sajeet" uniqKey="Haridas S" first="Sajeet" last="Haridas">Sajeet Haridas</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wu, Si" sort="Wu, Si" uniqKey="Wu S" first="Si" last="Wu">Si Wu</name>
<affiliation wicri:level="2"><nlm:affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington</wicri:regionArea>
<placeName><region type="state">Washington (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Labutti, Kurt" sort="Labutti, Kurt" uniqKey="Labutti K" first="Kurt" last="Labutti">Kurt Labutti</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name>
<affiliation wicri:level="2"><nlm:affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Henrissat, Bernard" sort="Henrissat, Bernard" uniqKey="Henrissat B" first="Bernard" last="Henrissat">Bernard Henrissat</name>
<affiliation wicri:level="3"><nlm:affiliation>Architecture et Fonction des Macromolécules Biologiques, UMR7257, Centre National de la Recherche Scientifique and Aix-Marseille Université, 13288 Marseille Cedex 9, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, UMR7257, Centre National de la Recherche Scientifique and Aix-Marseille Université, 13288 Marseille Cedex 9</wicri:regionArea>
<placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Department of Biological Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589</wicri:regionArea>
<wicri:noRegion>21589</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Santelli, Cara M" sort="Santelli, Cara M" uniqKey="Santelli C" first="Cara M" last="Santelli">Cara M. Santelli</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Earth Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Earth Sciences, University of Minnesota, Minneapolis, Minnesota</wicri:regionArea>
<placeName><region type="state">Minnesota</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Hansel, Colleen M" sort="Hansel, Colleen M" uniqKey="Hansel C" first="Colleen M" last="Hansel">Colleen M. Hansel</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, United States of America.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">PloS one</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (enzymology)</term>
<term>Biodegradation, Environmental (MeSH)</term>
<term>Carbon (metabolism)</term>
<term>Cations, Divalent (MeSH)</term>
<term>Cellulases (classification)</term>
<term>Cellulases (metabolism)</term>
<term>Computational Biology (MeSH)</term>
<term>Glycoside Hydrolases (classification)</term>
<term>Glycoside Hydrolases (metabolism)</term>
<term>Hydroxyl Radical (metabolism)</term>
<term>Lignin (metabolism)</term>
<term>Manganese (metabolism)</term>
<term>Molecular Sequence Annotation (MeSH)</term>
<term>Oxidation-Reduction (MeSH)</term>
<term>Polygalacturonase (classification)</term>
<term>Polygalacturonase (metabolism)</term>
<term>Soil Microbiology (MeSH)</term>
<term>Species Specificity (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Annotation de séquence moléculaire (MeSH)</term>
<term>Ascomycota (enzymologie)</term>
<term>Biologie informatique (MeSH)</term>
<term>Carbone (métabolisme)</term>
<term>Cations divalents (MeSH)</term>
<term>Cellulases (classification)</term>
<term>Cellulases (métabolisme)</term>
<term>Dépollution biologique de l'environnement (MeSH)</term>
<term>Glycosidases (classification)</term>
<term>Glycosidases (métabolisme)</term>
<term>Lignine (métabolisme)</term>
<term>Manganèse (métabolisme)</term>
<term>Microbiologie du sol (MeSH)</term>
<term>Oxydoréduction (MeSH)</term>
<term>Polygalacturonase (classification)</term>
<term>Polygalacturonase (métabolisme)</term>
<term>Radical hydroxyle (métabolisme)</term>
<term>Spécificité d'espèce (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Cellulases</term>
<term>Glycoside Hydrolases</term>
<term>Polygalacturonase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term>
<term>Cellulases</term>
<term>Glycoside Hydrolases</term>
<term>Hydroxyl Radical</term>
<term>Lignin</term>
<term>Manganese</term>
<term>Polygalacturonase</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Cellulases</term>
<term>Glycosidases</term>
<term>Polygalacturonase</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Ascomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Ascomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carbone</term>
<term>Cellulases</term>
<term>Glycosidases</term>
<term>Lignine</term>
<term>Manganèse</term>
<term>Polygalacturonase</term>
<term>Radical hydroxyle</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term>
<term>Cations, Divalent</term>
<term>Computational Biology</term>
<term>Molecular Sequence Annotation</term>
<term>Oxidation-Reduction</term>
<term>Soil Microbiology</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Annotation de séquence moléculaire</term>
<term>Biologie informatique</term>
<term>Cations divalents</term>
<term>Dépollution biologique de l'environnement</term>
<term>Microbiologie du sol</term>
<term>Oxydoréduction</term>
<term>Spécificité d'espèce</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the environment. While secretome studies of model organisms such as Phanerochaete chrysosporium and Aspergillus species have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates or conducted side-by-side comparisons of diverse species. Thus, the mechanisms of carbon degradation by many ubiquitous soil fungi remain poorly understood. Here we use a combination of LC-MS/MS, genomic, and bioinformatic analyses to characterize and compare the protein composition of the secretomes of four recently isolated, cosmopolitan, Mn(II)-oxidizing Ascomycetes (Alternaria alternata SRC1lrK2f, Stagonospora sp. SRC1lsM3a, Pyrenochaeta sp. DS3sAY3a, and Paraconiothyrium sporulosum AP3s5-JAC2a). We demonstrate that the organisms produce a rich yet functionally similar suite of extracellular enzymes, with species-specific differences in secretome composition arising from unique amino acid sequences rather than overall protein function. Furthermore, we identify not only a wide range of carbohydrate-active enzymes that can directly oxidize recalcitrant carbon, but also an impressive suite of redox-active accessory enzymes that suggests a role for Fenton-based hydroxyl radical formation in indirect, non-specific lignocellulose attack. Our findings highlight the diverse oxidative capacity of these environmental isolates and enhance our understanding of the role of filamentous Ascomycetes in carbon turnover in the environment. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27434633</PMID>
<DateCompleted><Year>2017</Year>
<Month>07</Month>
<Day>18</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>12</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>11</Volume>
<Issue>7</Issue>
<PubDate><Year>2016</Year>
</PubDate>
</JournalIssue>
<Title>PloS one</Title>
<ISOAbbreviation>PLoS One</ISOAbbreviation>
</Journal>
<ArticleTitle>Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.</ArticleTitle>
<Pagination><MedlinePgn>e0157844</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0157844</ELocationID>
<Abstract><AbstractText>Fungal secretomes contain a wide range of hydrolytic and oxidative enzymes, including cellulases, hemicellulases, pectinases, and lignin-degrading accessory enzymes, that synergistically drive litter decomposition in the environment. While secretome studies of model organisms such as Phanerochaete chrysosporium and Aspergillus species have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates or conducted side-by-side comparisons of diverse species. Thus, the mechanisms of carbon degradation by many ubiquitous soil fungi remain poorly understood. Here we use a combination of LC-MS/MS, genomic, and bioinformatic analyses to characterize and compare the protein composition of the secretomes of four recently isolated, cosmopolitan, Mn(II)-oxidizing Ascomycetes (Alternaria alternata SRC1lrK2f, Stagonospora sp. SRC1lsM3a, Pyrenochaeta sp. DS3sAY3a, and Paraconiothyrium sporulosum AP3s5-JAC2a). We demonstrate that the organisms produce a rich yet functionally similar suite of extracellular enzymes, with species-specific differences in secretome composition arising from unique amino acid sequences rather than overall protein function. Furthermore, we identify not only a wide range of carbohydrate-active enzymes that can directly oxidize recalcitrant carbon, but also an impressive suite of redox-active accessory enzymes that suggests a role for Fenton-based hydroxyl radical formation in indirect, non-specific lignocellulose attack. Our findings highlight the diverse oxidative capacity of these environmental isolates and enhance our understanding of the role of filamentous Ascomycetes in carbon turnover in the environment. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zeiner</LastName>
<ForeName>Carolyn A</ForeName>
<Initials>CA</Initials>
<AffiliationInfo><Affiliation>School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Purvine</LastName>
<ForeName>Samuel O</ForeName>
<Initials>SO</Initials>
<AffiliationInfo><Affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zink</LastName>
<ForeName>Erika M</ForeName>
<Initials>EM</Initials>
<AffiliationInfo><Affiliation>Biological Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Paša-Tolić</LastName>
<ForeName>Ljiljana</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chaput</LastName>
<ForeName>Dominique L</ForeName>
<Initials>DL</Initials>
<AffiliationInfo><Affiliation>Department of Mineral Sciences, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Haridas</LastName>
<ForeName>Sajeet</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wu</LastName>
<ForeName>Si</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>LaButti</LastName>
<ForeName>Kurt</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Grigoriev</LastName>
<ForeName>Igor V</ForeName>
<Initials>IV</Initials>
<AffiliationInfo><Affiliation>U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Henrissat</LastName>
<ForeName>Bernard</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Architecture et Fonction des Macromolécules Biologiques, UMR7257, Centre National de la Recherche Scientifique and Aix-Marseille Université, 13288 Marseille Cedex 9, France.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biological Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Santelli</LastName>
<ForeName>Cara M</ForeName>
<Initials>CM</Initials>
<AffiliationInfo><Affiliation>Department of Earth Sciences, University of Minnesota, Minneapolis, Minnesota, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hansel</LastName>
<ForeName>Colleen M</ForeName>
<Initials>CM</Initials>
<AffiliationInfo><Affiliation>Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts, United States of America.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>07</Month>
<Day>19</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>PLoS One</MedlineTA>
<NlmUniqueID>101285081</NlmUniqueID>
<ISSNLinking>1932-6203</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D002413">Cations, Divalent</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>11132-73-3</RegistryNumber>
<NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>3352-57-6</RegistryNumber>
<NameOfSubstance UI="D017665">Hydroxyl Radical</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>42Z2K6ZL8P</RegistryNumber>
<NameOfSubstance UI="D008345">Manganese</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>7440-44-0</RegistryNumber>
<NameOfSubstance UI="D002244">Carbon</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber>
<NameOfSubstance UI="D044602">Cellulases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber>
<NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber>
<NameOfSubstance UI="C023305">hemicellulase</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.2.1.15</RegistryNumber>
<NameOfSubstance UI="D011096">Polygalacturonase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002413" MajorTopicYN="N">Cations, Divalent</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044602" MajorTopicYN="N">Cellulases</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017665" MajorTopicYN="N">Hydroxyl Radical</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008345" MajorTopicYN="N">Manganese</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D058977" MajorTopicYN="N">Molecular Sequence Annotation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011096" MajorTopicYN="N">Polygalacturonase</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>02</Month>
<Day>26</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>06</Month>
<Day>06</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>7</Month>
<Day>20</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2016</Year>
<Month>7</Month>
<Day>21</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>7</Month>
<Day>19</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">27434633</ArticleId>
<ArticleId IdType="doi">10.1371/journal.pone.0157844</ArticleId>
<ArticleId IdType="pii">PONE-D-16-08372</ArticleId>
<ArticleId IdType="pmc">PMC4951024</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Appl Environ Microbiol. 2014 Apr;80(7):2062-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24441164</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2001 Jan 19;305(3):567-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11152613</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Mikrobiol. 1969;64(4):338-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5386174</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2014 Oct 31;5:5277</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25358478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2009 May;47(5):1596-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19297598</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Divers. 2012 Mar;53(1):1-221</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23097638</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>IUBMB Life. 2014 Feb;66(2):122-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24578277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2001 Sep;11(9):1484-502</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11544192</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2002 Jul;68(7):3514-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12089036</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Cell Fact. 2010 Apr 20;9:23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20406453</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Respir Crit Care Med. 1997 Apr;155(4):1356-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9105079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2013 Oct 16;6(1):149</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24131596</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2013 Oct;97(19):8683-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23948728</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2007;2(4):953-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17446895</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2003 Sep;13(9):2178-89</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12952885</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2000 Jul 21;300(4):1005-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10891285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int Microbiol. 2002 Jun;5(2):53-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12180781</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Chem. 2006 Nov 15;78(22):7796-801</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17105173</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1985 Oct;150(1):76-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3843705</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2014 Jan;42(Database issue):D699-704</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24297253</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Biochim Pol. 2003;50(1):81-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12673349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1997 Sep 19;277(5333):1802-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9295267</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genet. 2005 Dec;48(6):366-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16283313</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 1999 May 21;451(2):186-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10371162</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2014 Nov;72:124-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24915038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2011 Sep 29;8(10):785-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 1994 Aug;176(16):4838-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8050996</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2014 Aug 27;15:722</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25159997</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Rev. 2006 Mar;30(2):215-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16472305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2012 Jul 26;5(1):52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22835028</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Dec 26;8(12):e85532</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24386479</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2010 Aug 6;9(8):3932-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20545367</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1513-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21187386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Protein Sci. 1997 Mar;6(3):501-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9070434</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2010 Jul;76(14):4871-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20495049</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2012 Aug 08;13:380</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22873931</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1992 Nov 25;267(33):23688-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1429709</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Biochem Biophys. 1986 Dec;251(2):688-96</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3800395</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24270786</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2014 Nov 07;9(11):e112179</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25379782</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2010;604:55-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20013364</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microb Biotechnol. 2009 Mar;2(2):164-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21261911</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Dec 02;8(12):e81033</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24312521</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2005 Oct;5(15):3847-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16130174</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2010 Jun;76(11):3599-610</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20400566</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2011 May;11(10 ):2019-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21500348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Biochem Mol Biol. 2010;1(1):36-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21968746</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Biofuels. 2013 Mar 21;6(1):41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23514094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biotechnol. 2000 Mar 10;78(2):93-113</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10725534</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1996 Jan 12;271(2):681-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8557673</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2004 Mar;41(3):327-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14761793</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2006 May 1;7(3):147-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20507435</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2001 Jun;67(6):2705-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11375184</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2014 Jan;42(Database issue):D503-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24157837</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008 Feb 06;3(2):e1542</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18253490</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2013 Feb;13(3-4):597-608</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23349114</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Bioinformatics. 2008 Apr 1;24(7):1021-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18304935</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Arabie saoudite</li>
<li>France</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>District de Columbia</li>
<li>Massachusetts</li>
<li>Minnesota</li>
<li>Provence-Alpes-Côte d'Azur</li>
<li>Washington (État)</li>
</region>
<settlement><li>Cambridge (Massachusetts)</li>
<li>Marseille</li>
</settlement>
<orgName><li>Université Harvard</li>
</orgName>
</list>
<tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Zeiner, Carolyn A" sort="Zeiner, Carolyn A" uniqKey="Zeiner C" first="Carolyn A" last="Zeiner">Carolyn A. Zeiner</name>
</region>
<name sortKey="Chaput, Dominique L" sort="Chaput, Dominique L" uniqKey="Chaput D" first="Dominique L" last="Chaput">Dominique L. Chaput</name>
<name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name>
<name sortKey="Hansel, Colleen M" sort="Hansel, Colleen M" uniqKey="Hansel C" first="Colleen M" last="Hansel">Colleen M. Hansel</name>
<name sortKey="Haridas, Sajeet" sort="Haridas, Sajeet" uniqKey="Haridas S" first="Sajeet" last="Haridas">Sajeet Haridas</name>
<name sortKey="Labutti, Kurt" sort="Labutti, Kurt" uniqKey="Labutti K" first="Kurt" last="Labutti">Kurt Labutti</name>
<name sortKey="Pasa Toli, Ljiljana" sort="Pasa Toli, Ljiljana" uniqKey="Pasa Toli L" first="Ljiljana" last="Paša-Toli">Ljiljana Paša-Toli</name>
<name sortKey="Purvine, Samuel O" sort="Purvine, Samuel O" uniqKey="Purvine S" first="Samuel O" last="Purvine">Samuel O. Purvine</name>
<name sortKey="Santelli, Cara M" sort="Santelli, Cara M" uniqKey="Santelli C" first="Cara M" last="Santelli">Cara M. Santelli</name>
<name sortKey="Wu, Si" sort="Wu, Si" uniqKey="Wu S" first="Si" last="Wu">Si Wu</name>
<name sortKey="Zink, Erika M" sort="Zink, Erika M" uniqKey="Zink E" first="Erika M" last="Zink">Erika M. Zink</name>
</country>
<country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Henrissat, Bernard" sort="Henrissat, Bernard" uniqKey="Henrissat B" first="Bernard" last="Henrissat">Bernard Henrissat</name>
</region>
</country>
<country name="Arabie saoudite"><noRegion><name sortKey="Henrissat, Bernard" sort="Henrissat, Bernard" uniqKey="Henrissat B" first="Bernard" last="Henrissat">Bernard Henrissat</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000222 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000222 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PhanerochaeteV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:27434633
   |texte=   Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:27434633" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PhanerochaeteV1

This area was generated with Dilib version V0.6.37.
Data generation: Fri Nov 13 18:33:39 2020. Site generation: Fri Nov 13 18:35:20 2020

	Serveur d'exploration sur le phanerochaete
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le phanerochaete

Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.

Comparative Analysis of Secretome Profiles of Manganese(II)-Oxidizing Ascomycete Fungi.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki